ProntoSIL 300-3-C18H
 Part number:
0746K185PS030
 Dimension:
75 x 4.6 mm
 Eluent:
A: H2O/0.1% TFA 
B: ACN/H2O 95/5 (v/v)/0.1% TFA
 Gradient:
a) 29-48% B, 0-25 s;
    48-100%B, 25-65 s 
b) 29-48% B, 0-49 s; 
    48-100% B, 49-130 s 
c) 29-100% B, 0-360 s
 Flow:
a) 4 ml/min; 
b) 2 ml/min; 
c) 1 ml/min
 Detection:
UV 220 nm
 Temperature:
30 °C
 Injection:
5 µl
 Sample:
1: Ribonuclease A 
2: Insulin, bovine 
3: Lysozyme 
4: BSA 
5: Myoglobin 
6: Ovalbumin 
7: not identified