ProntoSIL 120-3-C18 AQ
 Part number:
2003F184PS030
 Dimension:
200 x 3.0 mm
 Eluent:
A: H2O/50 mM H3PO4 
B: ACN
 Gradient:
0% B, 0-180 s; 
0-30% B, 181-400 s; 
30 % B, 401-790 s
 Flow:
0.7 ml/min
 Detection:
UV 230 nm
 Temperature:
22 °C
 Injection:
5 µl
 Sample:
1: Pyridoxamine 
2: Thiamine (Vit. B1) + impurity 
3: Ascorbic acid (Vit. C) 
4: Nicotinic acid (Niacin) 
5: Nicotinamide (Vit. B3)
6: Pyridoxal 
7: Pyridoxine (Vit. B6)+ impurity of Vit. B12 
8: Folic acid 
9: Cyanocobalamin (Vit. B12) 
10: Riboflavin (Vit. B2)